Western blot analysis of TSC1 in extracts of HT-1080 cells. Samples were incubated with TSC1 Monoclonal antibody (Product # MA5-35652) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.
Western Blot analysis of TSC1 performed on various cell lines using a TSC1 monoclonal antibody (Product # MA5-35652) at a dilution of 1:1,000. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.
Supplier Page from Thermo Fisher Scientific for TSC1 Antibody